A Staphylococcus aureus count plate is a type of microbiological culture medium used to estimate the number of Staphylococcus aureus bacteria present in a sample. The procedure involves serial dilution of the sample and plating it on a specific agar medium that supports the growth of Staphylococcus aureus.
Here’s a general overview of the steps involved in conducting a Staphylococcus aureus count plate:
1. Sample collection: Obtain a sample suspected to contain Staphylococcus aureus, such as a swab from a wound or a sample from a food product.
2. Serial dilution: The sample is diluted multiple times to obtain different dilutions. This step helps to reduce the number of bacteria in the sample so that individual colonies can be counted accurately.
3. Plating: A small volume of each dilution is spread or poured onto a Petri dish containing a nutrient agar medium that promotes the growth of Staphylococcus aureus.
4. Incubation: The Petri dishes are incubated at an appropriate temperature, usually around 37°C (98.6°F), which allows the bacteria to grow and form visible colonies.
5. Colony counting: After incubation, the number of colonies on each plate is counted. The number of colonies is proportional to the number of bacteria in the original sample.
6. Calculation: By knowing the dilution factor and the number of colonies counted on the plate, it is possible to calculate the original Staphylococcus aureus count in the sample.
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